lympholyte human cell separation media  (Cedarlane)

Journal: Cell reports

Article Title: Staphylococcus aureus promotes strain-dependent immunopathology during cutaneous leishmaniasis through induction of IL-1β

doi: 10.1016/j.celrep.2025.115624

Figure Lengend Snippet: (A) Pathology scoring and ear thickness measurements in mice uninfected or infected with either L. major alone or with both S. aureus colonization and L. major infection (Wilcoxon, * p < 0.05 and ** p < 0.01; mean ± SD representative of 2 experiments; L. major-S. aureus treatment, IL-1β –/– mouse, n = 8 mice, and all other conditions , n = 6 mice). (B) Bacterial burden (CFUs) and L. major parasite burden at day 7 post- L. major infection (Wilcoxon, * p < 0.05). (C) Flow cytometric analysis of immune cell recruitment (Ly6G+, CD4 + , and CD8 + cells) in infected ear skin (Wilcoxon, * p < 0.05, ** p < 0.01, and *** p < 0.001; mean representative of 2 experiments; L. major-S. aureus treatment , IL-1β –/– mouse, n = 8 mice and all other conditions , n = 6 mice). (D) Percentage of immune cells recruited to the site of infection in mice infected with L. major with and without colonization of S. aureus . (E) Pie chart showing percentage of IL-1β+ cells that co-stain with neutrophils, macrophages, monocytes, and unidentified cells at 7 days post-infection. Based on flow cytometric data.

Article Snippet: Neutrophil isolation medium (Lympholyte-poly cell separation media) , Cedarlane , Cat#CL5070.

Techniques: Infection, Staining

Journal: Cell reports

Article Title: Staphylococcus aureus promotes strain-dependent immunopathology during cutaneous leishmaniasis through induction of IL-1β

doi: 10.1016/j.celrep.2025.115624

Figure Lengend Snippet: (A) Schematic outlining the study design of sample collection from patients with L. braziliensis infections. (B) Gene expression of IL-1β in lesion biopsies with an S. aureus -dominant or heterogeneous microbiome profile (Wilcoxon, ** p < 0.01). (C) Relative neutrophils in lesion biopsies as determined by cell estimation (XCell) (Wilcoxon, * p < 0.05). (D) GO pathway analysis comparing lesion biopsies with S. aureus dominance or a heterogeneous microbiome (Fisher’s one-tailed test with Benjamini-Hochberg adjustment, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

Article Snippet: Neutrophil isolation medium (Lympholyte-poly cell separation media) , Cedarlane , Cat#CL5070.

Techniques: Gene Expression, One-tailed Test

Journal: Cell reports

Article Title: Staphylococcus aureus promotes strain-dependent immunopathology during cutaneous leishmaniasis through induction of IL-1β

doi: 10.1016/j.celrep.2025.115624

Figure Lengend Snippet: (A) Schematic of bacterial swab extraction and isolate identification by MALDI-TOF. (B) Quantification of the frequency of bacterial species recovered from 44 human cutaneous leishmaniasis lesions. Each species is only counted once per lesion. (C) Phylogenetic tree of S. aureus clinical isolates and reference genomes. Layered onto the tree is each isolate’s methicillin resistance status and clonal complex. (D) ELISA quantifying levels of IL-1β recovered from mouse and human neutrophils infected with CLSA2 and CLSA50 strains (Wilcoxon, *** p < 0.001). (E) Bacterial survival of CLSA2 and CLSA50 in response to infection of human and mouse neutrophils for 1 h (paired t test, * p < 0.05). n = 4 human donors; n = 5 mice.

Article Snippet: Neutrophil isolation medium (Lympholyte-poly cell separation media) , Cedarlane , Cat#CL5070.

Techniques: Extraction, Enzyme-linked Immunosorbent Assay, Infection